Exjobbspresentation Sravan Ramachandran, Kemi och kemiteknik

Modification and Characterisation of Bactericidal Particles.​

Abstract:
Antibiotic resistance has been a global threat from the invention of first antibiotic. Because of this, alternative for antibiotics have been a major area of research. This projects circles around antimicrobial peptides (AMPs) which are an essential part of our innate immune system. AMPs are short peptide chains which targets foreign microbes entering the human body. The main feature of AMPs are that they kill the microbes at very low concentrations without affecting the viability of human cells. This is because of the neutral charge on the outer membrane of mammalian cell which doesn't trigger the electrostatic attraction of AMPs towards the cell. One major drawback of AMPs are that in presence of proteolytic enzyme, they have a very short lifetime due to proteolytic degradation. This has been successfully overcome by covalently attaching the AMPs onto Pluronic F127 surface by the ongoing researches in Amferia AB, which is a company in Sweden who focuses on developing a bactericidal wound patches. The focus of this master thesis was to develop different novel methods to synthesize the Pluronic hydrogel particles which can be modified using the AMPs and used as a wound spray to treat deep wound infections. Three methods were developed to synthesize the hydrogel particles. Spray polymerization, cloud point and the hot water method. After developing the protocol for synthesizing the hydrogel particles using these methods, they were tested with Staphylococcus epidermidis bacterial strain using agar plate modelling. The spray polymerized particles showed the highest activity followed by the hot water particles and cloud point particles failed to kill the bacteria. Other analysis tools like SEM (scanning electron microscopy) and SAXS (Small angle X-ray scattering) were performed to give more insight towards the morphology and order of the particles. By replacing the environment of crosslinking of the sprayed Pluronic gel to obtain spray polymerized particles, 50% loss of hydrogel was able to be brought down to 30%. This happens due to oxygen inhibition and in a crosslinking environment where there is no oxygen, this can be brought even further down.

Examiner : Martin Andersson

Supervisor : Edvin Blomstrand

Opponent : Aditya Venkatesh Ravirajan


Kategori Studentarbete
Plats: Kemihuset, Pater Noster, rum 2034
Tid: 2022-05-18 13:00
Sluttid: 2022-05-18 14:00

Sidansvarig Publicerad: to 12 maj 2022.