Developing efficient cell factories, able to produce new or traditional fermentation products at high productivities and yields is an imperative need for the industrial feasibility of bioprocesses. Our main focus is to use the yeast Saccharomyces cerevisiae as production host; we work to develop S. cerevisiae in a number of ways to
i) increase the substrate range to enable use of all biomass-derived sugars,
ii) expand the palette of target products
iii) improve the microbial robustness towards lignocellulose-derived inhibitors and process hardiness.
In concert to the extensive work on S. cerevisiae, we also characterize and develop non-conventional yeasts, filamentous fungi and bacteria as production hosts. We widely use omics analysis to characterize our strains. Furthermore, we put emphasis into genetic tool development for cell factory design. Here, we focus on accelerating the design-build-test-learn cycle, standardized strain engineering strategies and high throughput strain evaluation methodology, including use of biosensors. CRISPR/Cas9 based genome editing technologies and CRISPRi based alteration of gene transcription form core competences of our research efforts.
Ongoing centers and projects: