Industrial Biotechnology for Lignocellulose Based Processes
Chalmers University of Technology, Gothenburg, Sweden
October 27th– November 1st, 2019
Course Aims and Contents
The aim is to introduce the students to production of biofuels and other chemicals using plant cell wall materials as the raw material with emphasis on the biotechnology aspect of the production process.
Lectures and exercises will be mixed. The course also includes a poster session where participants present their research.
Download the course leaflet here. Who should attend?
The course is suitible to PhD students or researchers with a background in bio-technology, chemical engineering or biochemistry. Accredited with 5 ECTSpoints.
Course fee, accommodation
The course fee is 3.500 SEK (~330 €) for academic participants and 8.000 SEK (~840 €) for participants from the industry.
The course fee includes all course material, social events, amenities, 6 lunches and 3 dinners. Accommodation will be arranged for those requiring it (2.000 SEK, 26th of October – 1st of November, in 4 or 5-bedrooms, at Göteborgs Vandrarhem (Gothenburg Hostel).
Registration and information
Course Program – Preliminary Schedule
Sunday October 27th
Introduction, raw material composition and sources
09:00-09:30 Course introduction. Round the table presentations (LO)
09:30-10:15 Lignocellulose conversion to chemicals – from stand-alone facilities to biorefineries
(LO)
10:15-10:30 Coffee
10:30-11:15 Plant cell wall fundamentals (JL)
11:15-12:00 Basic raw material analytics: HPLC, HPAEC, reducing sugars (LO)
12:00-13:00 Lunch – 10:an
13:00-14:00 Fractionation and pre-treatment methods (MG)
14:00-15:00 Mass spectrometric-based glycomic analysis of lignocellulosic materials (FVF)
15:00-15:30 Coffee
15:30-16:15 Inhibitors in lignocellulose hydrolysates (LO)
16:15-17:00 Enzyme discovery for biorefinery applications (JL)
17:00-17:45 Exercises and discussion x 2
18:00 Dinner at Chalmers, student poster presentation, get-together
Monday October 28th
Characterization & enzymatic degradation of lignocelluloses
09:00-09:45 Challenges in sample preparation and analysis of lignocellulose polymeric materials
(MH)
09:45-10:00 Coffee
10:00-11:00 Enzymatic hydrolysis of lignocellulose - introduction (LO/JL)
11:00-11:45 Exercises and discussion x 2
12:00-13:00 Lunch - kårhuset
13:00-13:45 Cellulolytic enzymes (LO)
13:45-14:45 Mannolytic and xylanolytic enzymes
14:45-15:15 Coffee
15:15-16:00 Bacterial anaerobic degradation of biomass (JL)
16:00-16.45 Advanced enzyme technologies (KSJ)
16:45-17:30 Exercises and discussion x 2
Tuesday October 29th
Enzymes and their action & Hot topics seminar
09:00-10:00 Lytic Polysaccharide Monooxygenases (LPMOs) and other redox enzymes in polysaccharide degradation – fundamental and applied aspects (VE)
10:00-10:15 Coffee
10:15-11.15 Kinetics and design of cellulases (PW)
11:15-12:00 Exercise: Enzyme classification & CAZY (JL)
12:00-13:00 Lunch – 10:an
13:00-13:20 HOT topics intro (LO)
13:20-14:00 Phillip Pope: Wood2Feed: Can we use woody biomass as feed ingredients and improve diets in livestock?
14:00-14:40 Richard van Kranenburg: Bacterial cell factories for the biobased economy
14:40-15:10 Coffee
15:10-15:50 Emma Master – TBD
15:50-16:30 Björn Alriksson – Processum: From wood to food
16:30-17.10 Kaspar Valgepea - Capturing waste carbon using acetogen gas fermentation
18:30 Course Dinner downtown Gothenburg M/S Marieholm
Wednesday October 30th
Enzymes & Microorganisms
09:00-10:00 TBD
10:00-10:15 Coffee
10:15-11:15 Lignin acting enzymes (KH)
11:15-12:00 Exercises and discussion x 2 YN+CG
12:00-13:00 Lunch
13:00-13:45 Different products of microbial metabolism and thermodynamic driving forces (YN)
13:45-14:30 Strain improvement by mutagenesis, directed evolution and screening (CG)
14:30-15:00 Coffee
15:00-15:45 Strain improvement by metabolic engineering (YN)
15:45-16.30 Development of industrial yeast strains (YN)
16:30-17.30 Exercises and discussion x 2 (YN+CG)
Thursday October 31st
Fermentation processes
09:00-09:45 Cell propagation (LO)
09:45-10:30 Lignocellulose hydrolysate fermentation: microbial robustness (LO)
10:30-10:45 Coffee
10:45-11:45 Quantitative evaluation of fermentations of lignocellulose hydrolysate - exercise and discussion
12:00-13:00 Lunch, kårhuset
13:00-13:45 Fermentation basics: important fermentation variables and mode of operation of bioreactors (CJF)
13:45-14:30 Fermentation basics: important fermentation variables and mode of operation of bioreactors - continued (CJF)
14:30-15:00 Coffee
15:00-15:45 Fermentation of lignocellulosic media: mode of operation and strategies (CJF)
15:45-16.45 TBD – microbial detoxification
16:45-17.30 Exercises and discussion x 2 (CJF, LO)
17:30-18.00 Concluding discussions, questions and sum-up
18:00 Pizza at the department and lab visit
Friday November 1st
Sustainability, down-stream processing etc.
Exam
09:00-09:45 Enzyme production by filamentous fungi: from secretion to large scale production (LO)
09:45-10:45 Bioenergy and biomaterials sustainability issues (CC)
10:45-11:00 Coffee
11:00-12:00 Down-stream processing (IS)
12:00-13:00 Lunch – 10:an
13:00-15:00 Written exam
Good-bye and wrap up over coffee
Contacts
Confirmed course lecturers
Aalto University, Espoo, Finland
Emma Master
Chalmers University of Technology; Gothenburg, Sweden
Lisbeth Olsson, Carl Johan Franzén, Cecilia Geijer, Johan Larsbrink, Yvonne Nygård, Christel Cederberg, Merima Hasani
Corbion, Wageningen University and Research, The Netherlands
Richard van Kranenburg
Lund University, Sweden
Mats Galbe
Norwegian University of Life Sciences, Ås, Norway
Vincent Eijsink, Phillip Pope
RISE Processum AB, Örnsköldsvik, Sweden
Björn Alriksson
Royal Institute of Technology, Stockholm, Sweden
Francisco Xavier Vilaplana Domingo
Technical University of Denmark
Ioannis Kiadas, Peter Westh
University of Copenhagen, Denmark
Katja Salomon Johansen
University of Helsinki, Finland
Kristiina Hildén
University of Tartu, Estonia
Kaspar Valgepea
