Befordransföreläsning: Florian David

Florian David ger sin föreläsning för befordran till docent.

Sammanfattning:

In recent years the discipline of Biomolecular Engineering has seen many advances in the development of novel methodologies to speed up Design-Build-Test-Learn (DBTL) cycles in various areas ranging from cell factory development to drug discovery applications.
To name a few, CRISPR/Cas based tools have enabled more efficient and precise genetic engineering, intracellular biosensors have facilitated high throughout screenings and next generation sequencing technologies allowed for detailed understanding of underlaying genetic traits. A key tool in the DBTL cycle for protein engineering is the Nobel Prize-winning methodology of directed evolution. In the last years in vivo mutagenesis tools have been developed to further advance this technique by allowing for more continuous and in-depth exploration of the genetic sequence space.

One of the key microbial hosts used in Biomolecular Engineering and Synthetic Biology is baker's yeast, Saccharomyces cerevisiae. It is a well characterized eukaryotic model organism and industrially established and robust production host. At the same time, it is a model for various human related diseases making it also a suitable tool for drug discovery applications.

Within this lecture I will highlight the above and future developments through specific examples from our own research including the development of synthetic biology tools, in vivo mutagenesis approaches and high throughput screenings of engineered baker´s yeast as microbial cell factory and advanced drug discovery platform.