Lipid Nanoparticle Generation and Characterization

​Lead academic principle investigator: Fredrik Höök (Chalmers),
Industrial and academic partners: Lennart Lindfors and Annette Bak (AstraZeneca), Marcus Wilhelmsson (Chalmers), Vironova, Nanolyze, Camurus.

RNA Synthesis, Fluorescence-based Probe Design and Characterization

​Lead academic principal investigator: Marcus Wilhelmsson (Chalmers),
Industrial and academic partners: Malin Lemurell (AstraZeneca), Elin Esbjörner (Chalmers), Hadi Valadi (University of Gothenburg), Fredrik Höök (Chalmers), Samir El-Andaloussi (Karolinska Institutet), Lennart Lindfors, Anna Collén.

Exosome Biology and RNA-loading

​Lead academic principal investigator: Samir El-Andaloussi (Karolinska Institutet)
Industrial and academic partners: Anna Collén and Per-Erik Strömstedt (AstraZeneca), Elin Esbjörner and Marcus Wilhelmsson (Chalmers).

Quantification of Endosomal Escape and Functional Delivery

​Lead academic principal investigator: Hadi Valadi (University of Gothenburg)
Industrial and Academic partners: Samir El-Andaloussi (Karolinska Institutet), Lennart Lindfors (AstraZeneca), Marcus Wilhelmsson, Elin Esbjörner and Fredrik Höök (Chalmers) and Camurus.

Novel Imaging Tools and Cellular Particle Tracking

​Lead academic principal investigators: Elin Esbjörner and Fredrik Höök (Chalmers)
Industrial and academic partners: Marcus Wilhelmsson (Chalmers), Anna Collén and Shalini Andersson (AstraZeneca), Samir El-Andaloussi and Molly Stevens (Karolinska Institutet).

Cellular Uptake and Intracellular Trafficking Mechanisms

​Lead academic principal investigator: Elin Esbjörner (Chalmers)
Industrial and academic partners: Anna Collén, Shalini Andersson and Lennart Lindfors (AstraZeneca), Samir El-Andaloussi (Karolinska Institutet), Fredrik Höök and Marcus Wilhelmsson (Chalmers).

Publications

2022

Growth media conditions influence the secretion route and release levels of engineered extracellular vesicles. Bost J, Saher O, Hagey D, Mamand D, Liang X, Zheng W, Corso G, Gustafsson O, Görgens A, Smith CIE, Zain R,  EL Andaloussi S and Gupta D.
Adv Healthcare Materials  (2022) 11(5)

Cell-specific targeting of extracellular vesicles through engineering the glycocalyx. Zheng W, He R, Liang X, Roudi S, Bost J, Coly P, Niel G, and EL Andaloussi S.
J Extracellular Vesicles (2022) 11(12)

Identification of storage conditions stabilizing extracellular vesicles preparations. Görgens A, Corso C, Hagey D, Jawad Wiklander R, Gustafsson M, Felldin U, Lee Y, Bostancioglu B, Sork H, Liang X, Zheng W, Mohammad D, van de Wakker S, Vader P, Zickler AM, Mammand DR, Ma L, Holme M, Stevens M, Wiklander OPB, EL Andaloussi S.
J Extracellular Vesicles (2022) 11(6):e12238

Novel endosomolytic compounds enable highly potent delivery of antisense oligonucleotides. Bost J, Ojansivu M, Munson M, Wesen E, Gallud A, Gupta D, Gustafsson O, Saher O, Rädler J, Higgins S, Lehto T, Holme M, Dahlen A, Engkvist O, Strömstedt PE, Andersson S, Smith CIE, Stevens M, Esbjörner E, Collen A and EL Andaloussi S.
Nature Comm Biology (2022) 5(1): 185

Peptide-Folding Triggered Phase Separation and Lipid Membrane Destabilization in Cholesterol-Rich Lipid Vesicles. Johanna Utterström, Hanna M. G. Barriga, Margaret N. Holme*, Robert Selegård, Molly M. Stevens and Daniel Aili.
Bioconjugate Chem. 2022, 33, 4, 736–746

Coupling Lipid Nanoparticle Structure and Automated Single-Particle Composition Analysis to Design Phospholipase-Responsive Nanocarriers. Hanna M. G. Barriga, Isaac J. Pence, Margaret N. Holme, James J. Doutch, Jelle Penders, Valeria Nele, Michael R. Thomas, Marta Carroni, Molly M. Stevens
Advanced Materials, 2022, 34, 26, 2200839

Interaction Kinetics of Individual mRNA-Containing Lipid Nanoparticles with an Endosomal Membrane Mimic: Dependence on pH, Protein Corona Formation, and Lipoprotein Depletion. Aliakbarinodehi N, Gallud A, Mapar M, Wesén E, Heydari S, Jing Y, Emilsson G, Liu K, Sabirsh A, Zhdanov VP, Lindfors L, Esbjörner EK, Höök F.
ACS Nano. 2022; 16(12):20163-20173

Lipid Nanoparticles Deliver the Therapeutic VEGFA mRNA In Vitro and In Vivo and Transform Extracellular Vesicles for Their Functional Extensions. Nawaz M, Heydarkhan-Hagvall S, Tangruksa B, González-King Garibotti H, Jing Y, Maugeri M, Kohl F, Hultin L, Reyahi A, Camponeschi A, Kull B, Christoffersson J, Grimsholm O, Jennbacken K, Sundqvist M, Wiseman J, Bidar AW, Lindfors L, Synnergren J, Valadi H.
Advanced Science. 2023 Feb 19:e2206187.

Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease. Franco C, Ghirardello A, Bertazza L, Gasparotto M, Zanatta E, Iaccarino L, Valadi H, Doria A, Gatto M.
Int. J. Mol. Sci. 2023 Feb 11;24(4):3663.

Review articles

Exploiting the biogenesis of EVs for bioengineering and therapeutic cargo loading. Rädler J, Gupta D, Zickler A, and EL Andaloussi S.
Mol Therapy. (2023)

Conference presentations

The PIs in the center has been invited to present the research results at several prestigious conferences in 2022, e.g. International Society of Cellular Therapies (ISCT), May 2022, San Francisco; American Society of EVs (ASMEV), Oct 2022, Asilomar, SF; Select Bio Extracellular vesicles and nanoparticle therapeutics conference, Oct 2022, Rotterdam; German/Austrian EV society conference, Oct 2022, Vienna; Gordon research conference, RNA Nanotechnology meeting, Jan 2023, Ventura, CA; Cold Spring Harbor Labs Nucleic Acids Therapeutics meeting, March 2023, Long Island NY; 8th NovAliX Conference Biophysics in Drug Discovery; Scandinavian Society for Biomaterials meeting in Jurmala, Latvia, 13-15 June 2022; BESS 2022, Lipid Bilayers at the ESS.