To date, four companies (AstraZeneca, Camurus, Vironova and Nanolyze) collaborate with the academic partners (Chalmers University of Technology, Karolinska Institutet and University of Gothenburg) through the Formulaex centre in six interconnected projects.
Lipid Nanoparticle Generation and Characterization
Lead academic principle investigator: Fredrik Höök (Chalmers),
Industrial and academic partners: Lennart Lindfors and Annette Bak (AstraZeneca), Marcus Wilhelmsson (Chalmers), Vironova, Nanolyze, Camurus.
RNA Synthesis, Fluorescence-based Probe Design and Characterization
Lead academic principal investigator: Marcus Wilhelmsson (Chalmers),
Industrial and academic partners: Malin Lemurell (AstraZeneca), Elin Esbjörner (Chalmers), Hadi Valadi (University of Gothenburg), Fredrik Höök (Chalmers), Samir El-Andaloussi (Karolinska Institutet), Lennart Lindfors, Anna Collén.
Exosome Biology and RNA-loading
Lead academic principal investigator: Samir El-Andaloussi (Karolinska Institutet)
Industrial and academic partners: Anna Collén and Per-Erik Strömstedt (AstraZeneca), Elin Esbjörner and Marcus Wilhelmsson (Chalmers).
Quantification of Endosomal Escape and Functional Delivery
Lead academic principal investigator: Hadi Valadi (University of Gothenburg)
Industrial and Academic partners: Samir El-Andaloussi (Karolinska Institutet), Lennart Lindfors (AstraZeneca), Marcus Wilhelmsson, Elin Esbjörner and Fredrik Höök (Chalmers) and Camurus.
Novel Imaging Tools and Cellular Particle Tracking
Lead academic principal investigators: Elin Esbjörner and Fredrik Höök (Chalmers)
Industrial and academic partners: Marcus Wilhelmsson (Chalmers), Anna Collén and Shalini Andersson (AstraZeneca), Samir El-Andaloussi and Molly Stevens (Karolinska Institutet).
Cellular Uptake and Intracellular Trafficking Mechanisms
Lead academic principal investigator: Elin Esbjörner (Chalmers)
Industrial and academic partners: Anna Collén, Shalini Andersson and Lennart Lindfors (AstraZeneca), Samir El-Andaloussi (Karolinska Institutet), Fredrik Höök and Marcus Wilhelmsson (Chalmers).
Publications 2023-2024
2023
Surface display of functional moieties on extracellular vesicles using lipid anchors. Zheng W, Schurz M, Jawad R, Gustafsson O, Gupta D, Slovak R, Traista A, Coluzzi A, Roudi S, Barone A, Farcas D, Kyriakopoulou E, Galli V, Sharma H, Meisner-Kober N, Honcharenko M, and EL Andaloussi S.
J Control Release (2023) 357; 630-40
Identification of novel scaffold proteins for improved endogenous engineering of extracellular vesicles. Zheng W, Rädler J, Sork H, Zheyu N, Roudi S, Bost J, Görgens A, Zhao Y, Mamand D, Liang X, Wiklander P, Lehto T, Gupta D, Nordin J and EL Andaloussi S.
Nature Communications (2023) 14(1): 4734
The cellular response to extracellular vesicles is dependent on their source and dose. Hagey D, Ojansivu M, Bostancioglu B, Saher O, Bost J, Gustafsson M, Gramignoli R, Svahn M, Gupta D, Stevens M, Görgens A, and EL Andaloussi S.
Science Advances (2023) 9(35):1168
Exploiting the biogenesis of EVs for bioengineering and therapeutic cargo loading. Rädler J, Gupta D, Zickler A, and EL Andaloussi S.
Mol Therapy. (2023) 31(5): 1231-50
Lipid Nanoparticles Deliver the Therapeutic VEGFA mRNA In Vitro and In Vivo and Transform Extracellular Vesicles for Their Functional Extensions. Nawaz M, Heydarkhan-Hagvall S, Tangruksa B, González-King Garibotti H, Jing Y, Maugeri M, Kohl F, Hultin L, Reyahi A, Camponeschi A, Kull B, Christoffersson J, Grimsholm O, Jennbacken K, Sundqvist M, Wiseman J, Bidar AW, Lindfors L, Synnergren J, Valadi H.
Advanced Science. 2023 Feb 19:e2206187.
DOI: 10.1002/advs.202206187
Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease. Franco C, Ghirardello A, Bertazza L, Gasparotto M, Zanatta E, Iaccarino L, Valadi H, Doria A, Gatto M.
Int. J. Mol. Sci. 2023 Feb 11;24(4):3663.
DOI: 10.3390/ijms24043663
Multiphoton characterization and live cell imaging using fluorescent adenine analogue 2CNqA. Jesper R. Nilsson, Carlos Benitez-Martin, Henry G. Sansom, Pauline Pfeiffer, Tom Baladi, Hoang-Ngoan Le, Anders Dahlén, Steven W. Magennis, and L. Marcus Wilhelmsson.
Physical Chemistry Chemical Physics, 25, 20218-20224, 2023
Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control. Mapar, M.; Sjöberg, M.; Zhdanov, V. P.; Agnarsson, B.; Höök, F.
Biomed. Opt. Express 2023, 14 (8), 4003-4016, Article.
DOI: 10.1364/boe.490051.
The In Vivo Fate of Polycatecholamine Coated Nanoparticles Is Determined by a Fibrinogen Enriched Protein Corona. Emilsson, G.; Liu, K.; Höök, F.; Svensson, L.; Rosengren, L.; Lindfors, L.; Sigfridsson, K.
ACS Nano 2023, 17 (24), 24725-24742, Article.
DOI: 10.1021/acsnano.3c04968.
2024
Synthesis and photophysical characterization of a pH-sensitive quadracyclic uridine (qU) analogue. Dr. Hoang-Ngoan Le, Dr. Jagannath Kuchlyan, Dr. Tom Baladi, Prof. Bo Albinsson, Dr. Anders Dahlén, Prof. L. Marcus Wilhelmsson.
Chemistry Europe (online). 17 January 2024.
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Welsh JA, Goberdhan DCI, O'Driscoll L, Buzas EI, Blenkiron C,… Valadi H. ... Théry C, Witwer KW.
J Extracell Vesicles. 2024 Feb;13(2):e12404.
DOI: 10.1002/jev2.12404.
Time Resolved Inspection of Ionizable-Lipid Facilitated Lipid Nanoparticle Disintegration and Cargo Release at an Endosomal Membrane Mimic. Aliakbarinodehi, N.; Niederkofler, S.; Olsén, E.; Jing, Y.; Emilsson, G.; Sjöberg, M.; Agnarsson, B.; Lindfors, L.; Höök, F.
bioRxiv 2024.
DOI: 10.1101/2024.02.22.580934.
Review articles
Exploiting the biogenesis of EVs for bioengineering and therapeutic cargo loading. Rädler J, Gupta D, Zickler A, and EL Andaloussi S.
Mol Therapy. (2023)
Conference presentations
The PIs in the center has been invited to present the research results at several prestigious conferences in 2022, e.g. International Society of Cellular Therapies (ISCT), May 2022, San Francisco; American Society of EVs (ASMEV), Oct 2022, Asilomar, SF; Select Bio Extracellular vesicles and nanoparticle therapeutics conference, Oct 2022, Rotterdam; German/Austrian EV society conference, Oct 2022, Vienna; Gordon research conference, RNA Nanotechnology meeting, Jan 2023, Ventura, CA; Cold Spring Harbor Labs Nucleic Acids Therapeutics meeting, March 2023, Long Island NY; 8th NovAliX Conference Biophysics in Drug Discovery; Scandinavian Society for Biomaterials meeting in Jurmala, Latvia, 13-15 June 2022; BESS 2022, Lipid Bilayers at the ESS.