Course overview
- Course codeFBOM116
- ECTS credits5
- DepartmentARCHITECTURE AND CIVIL ENGINEERING
- Graduate schoolCivil and Environmental Engineering
- Starts2026-03-02
- Ends2026-03-23
- PeriodicityCourse is normally given every two years in January/February. Since 2020 it has run fully online. The course was given recently in 2024.
- LanguageEnglish
- ApplicationContact the course coordinator
Course coordinator
- Kathleen Murphy
- Professor, Water Environment Technology, Architecture and Civil Engineering
About the course
PARAllel FACtor analysis (PARAFAC) is used to interpret natural organic matter (NOM) fluorescence when measured using excitation emission matrix (EEM) spectroscopy. PARAFAC resolves overlapping fluorescence signals into independent spectra representing different chemical components. When fluorescence signals conform to Beers Law, this process can lead to the identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This course will provide instruction on best-practice application of PARAFAC to fluorescence datasets using the drEEM toolbox for MATLAB.
This course is offered through the PhD program in Water Environment Technology at the Department of Architecture and Civil Engineering, Chalmers University of Technology. Participants who complete the course including submitting three assignments can obtain official credit (5 ETS points, Pass/Fail grade).
Topics to be covered
Prerequisites
An introductory MATLAB course or equivalent experience.
Obtaining course credit
To pass the course, you will need to submit three assignments by final deadline.
Location
This is an online course consisting of recorded lectures and in person discussions on Zoom. Zoom meetings are held on Mondays 8-10 am and repeated at 4-6 pm (Stockholm Time), to enable participation by students in a wide range of time zones.
The course is offered in collaboration with the Technical University of Denmark (DTU).
This course is offered through the PhD program in Water Environment Technology at the Department of Architecture and Civil Engineering, Chalmers University of Technology. Participants who complete the course including submitting three assignments can obtain official credit (5 ETS points, Pass/Fail grade).
Topics to be covered
- Flourescence and PARAFAC theory
- Acquiring, importing av validating flourescence measurements
- Data organisation
- Checking, cleaning and preparing datasets
- Inner filter correction
- Modelling with PARAF
- Diagnosing and validating PARAFAC models
Prerequisites
An introductory MATLAB course or equivalent experience.
Obtaining course credit
To pass the course, you will need to submit three assignments by final deadline.
Location
This is an online course consisting of recorded lectures and in person discussions on Zoom. Zoom meetings are held on Mondays 8-10 am and repeated at 4-6 pm (Stockholm Time), to enable participation by students in a wide range of time zones.
The course is offered in collaboration with the Technical University of Denmark (DTU).
More information
Enquiries: Kate Murphy
murphyk@chalmers.se
Literature
- Murphy KR, Stedmon CA, Wenig P, Bro R. OpenFluor- an online spectral library of auto-fluorescence by organic compounds in the environment. Analytical Methods. 2014; 6(3):658-61.
- Murphy KR, Stedmon CA, Graeber D, Bro R. Fluorescence spectroscopy and multi-way techniques. PARAFAC. Analytical Methods. 2013; 5(23):6557-66.
Lecturer
Teachers: Kathleen Murphy (Chalmers University of Technology), Urban Wünsch and Colin Stedmon (Danish Technical University)