Smalltalks "De novo assembly of yeast genomes using optical genome mapping"

Abstract: 

Optical genome mapping (OGM) is a technique that can facilitate genome assemblies and detect structural variations in genomes, overcoming limitations of traditional techniques. In this study, we use a nanofluidic device and competitive binding labeling to stretch and label DNA for its imaging. Computational methods are then used to de novo assemble the genomes of two model yeasts. Our results demonstrate the ability of OGM to extend genome coverage in comparison to traditional genome assemblies.