Unravelling the functions of new
antibiotic resistance genes
Projektförslag för kandidatarbete inom inst. Kemi och kemiteknik och Biologi och bioteknik
Avdelningen för Kemisk biologi
Institutionen för Biologi och Bioteknik, Chalmers tekniska högskola Bakgrund
Bacteria have a number of stress
response systems that help them cope with external stress. Several of those are
of relevance as resistance factors against medically used antibiotics. In a previous
study, we have profiled the proteomic stress response of the model bacterium Bacillus
subtilis in response to a number of antibiotics that target the bacterial cell
envelope. The cell envelope, composed of the cytoplasmic membrane and the
peptidoglycan cell wall, is a highly relevant antibiotic target, since
resistance against compounds targeting this structure is scarce. In our study,
we have found several new stress response systems that specifically responded
to either membrane or cell wall stress, or in some cases both. The function of
these systems is entirely unknown and the subject of this project.
In this Bachelor project, you
will work on six stress response systems that appeared as markers in our proteomic
study. Your task will be to find first evidence for their function under
antibiotic stress. To this end, you will characterize the phenotypes of the
corresponding deletion mutants under different stress conditions, e.g.
treatment with antibiotics that target the cell envelope on different levels.
You will also examine the localization of selected stress proteins within
bacterial cells using fluorescence microscopy. Moreover, you will analyze cell
envelope physiology in the deletion mutants with respect to cell envelope
integrity using fluorescence microscopic and spectroscopic techniques.
Genomförande /Viktiga moment/teknikinnehåll
In this project, you will
1. Use molecular cloning to
generate the relevant bacterial strains.
2. Characterize the phenotypes of
deletion mutants with regard to effects on growth rate, antibiotic sensitivity,
and cell envelope physiology.
3. Characterize the localization
of selected stress response proteins within bacterial cells using fluorescent fusion
Students should have general
laboratory experience (pipetting, making solutions, calculating dilutions). A
biology background is advantageous. Knowledge about microbiology and working
under sterile conditions is a plus but can as well be learned during the
project. Verbal communication during the project will be in English, but all writing
will be in Swedish.
Möjlig målgrupp: Bioteknik, Bt
Gruppstorlek: 4–6 studenter
Övriga handledare: Luna Baruah