This project gains inspiration from the fact that endosomal escape is a very poorly understood step in cytosolic delivery of RNA following endocytosis, in particular due to the fact that detecting delivered (cytosolic) RNA molecules by conventional intensity-based imaging is difficult because of the significantly stronger intensity from RNA remaining trapped in endosomes. We will here work with fluorescence-based assays that distinguishes RNA entrapped in endosomes from RNA released in the cytosol based on spectral shifts, FRET or lifetime changes, which all have the potential to provide considerably better contrast than intensity-based imaging.
Lead academic principal investigators: Elin Esbjörner and Fredrik Höök
Industrial and academic partners: Marcus Wilhelmsson
(Chalmers), Anna Collén and Shalini Andersson (AstraZeneca), Samir El-Andaloussi and Molly Stevens (Karolinska Institutet).