Projektförslag för kandidatarbete inom inst. Kemi och kemiteknik och
Biologi och bioteknik
Mixed-mode chromatography for the analysis of pharmaceutical analytes dissolved in neat
or high content of organic solvent
Avdelningen för Analytisk
kemi, Institutionen för Kemi och Kemiteknik, Chalmers tekniska högskola
Bakgrund
The pharmaceutical industry is more and more using automated
sample preparation and analysis in
drug development. In some cases, methanol is used as a dissolving medium which doesn’t work well with standard
reversed phase HPLC systems. A promising solution
is to use a mixed mode column, which is packed with particles enabling hydrophobic as well as ion exchange
interactions at the same time. The objective of this project is to investigate chromatographic parameters suitable
for this mixed mode strategy. This
involves testing e.g., mobile phase composition and gradient elution settings, different separation media,
separation selectivity and analyte detectability as well as optimizing analysis time for high sample throughput. If
successful, this could lead to
easier automation and higher reproducibility of the separations as well as a
more environmentally friendly
approach with less waste.
Problembeskrivning
Investigate chromatographic parameters suitable for mixed mode strategy.
Genomförande /Viktiga moment/teknikinnehåll
1.
Introduction and HPLC basics, literature studies
2. Screening of chromatographic parameters
3. Optimization and application for selected pharmaceutical compounds
Speciella förkunskapskrav: Analytisk Kemi kam010, önskvärt
med ej krav
Möjlig målgrupp: K, Kf och Bt
Gruppstorlek: 4-6 studenter
Huvudhandledare: Per Malmberg
Övriga handledare: Jan-Olof Svensson och Anna Granfors (AstraZeneca)
Studentinitierat projekt: Elin Allgén, Hannes
Sellén.