Hoda Mashadi Fathali

PhD student; Chemistry and Chemical Engineering, Analytical Chemistry

My primary project is aimed to quantify the effect of functionalized nanoparticles on cell communication by exocytosis. Exocytosis is based on the fusion of synaptic vesicles filled with a neurotransmitter, like dopamine, to the cell membrane and subsequent release of neurotransmitter molecules. Vesicle fusion with partial release has been demonstrated for both small synaptic vesicles and large dense core vesicles. In our group, amperometric measurements on PC12 cells have been carried out and the results indicate closing of vesicles after incomplete release of its contents, shown by the presence of “post spike feet”.
I’m interested to study the effect of nanotoxicity on partial fusion in exocytosis, by incubating the cells with gold nanoparticles and amperometry measurement of dopamine release from PC12 cells and close evaluation by subsequent use of transmission electron microscopy.
In my second project in collaboration with Ann-Sofie’s group, the idea is to synthesize and characterize chromogranin A nanoparticles. Chromogranin A (CgA) is single polypeptide chain protein that is mainly located within the polypeptide matrix of large dense core vesicles (LDCVs). And it is mainly the main protein component of the intravesicular matrix. An important property of CgA is thought to be the accumulation of large amounts of neurotransmitter within the vesicles, which can have an important role in exocytosis. In this project I’m interested to create a model of the polypeptide matrix found in large dense core vesicles by synthesizing and characterizing chromogranin A nanoparticles. I plan to study the interaction of dense core chromatogranin A nanoparticles with the lipid membrane and neurotransmitters like dopamine, epinephrine, and norepinephrine to provide insight into the role of the polypeptide matrix in exocytosis.

Published: Thu 19 Jan 2012. Modified: Tue 13 Jan 2015