Female Investigators in Nonlinear Optical Nanoscopy - FINON (FINON)

Develop Nonlinear Optical Microscopy (NOM) techniques into the nano-dimension, allowing for label-free three-dimensional (3D), dynamic imaging of macromolecular arrangements and nanostructures by probing inherent higher-order electronic and molecular vibrations with novel detection concepts. The FINON inititial training network constitutes of partners from, and active interaction between, a wide range of disciplines spanning from front-line technology developers of laser instrumentation, microscopy, and nanophotonics to users of microscopy with extended experience and challenging requirements within the life-, chemical-, pharmaceutical- and material sciences.

Partner organizations

  • AstraZeneca AB (Private, Sweden)
  • Institut Fresnel (Research Institute, France)
  • Cardiff University (Academic, United Kingdom)
  • Max Planck Institute (Research Institute, Germany)
  • Ecole Polytechnique Federale De Lausanne (Private, Switzerland)
Start date 01/10/2013
End date The project is closed: 30/09/2017


Female Investigators in Nonlinear Optical Nanoscopy

 
Develop Nonlinear Optical Microscopy (NOM) techniques into the nano-dimension, allowing for label-free three-dimensional (3D), dynamic imaging of macromolecular arrangements and nanostructures by probing inherent higher-order electronic and molecular vibrations with novel detection concepts. The FINON inititial training network constitutes of partners from, and active interaction between, a wide range of disciplines spanning from front-line technology developers of laser instrumentation, microscopy, and nanophotonics to users of microscopy with extended experience and challenging requirements within the life-, chemical-, pharmaceutical- and material sciences.
Focus:
  • Hierarchical structures of amyloid peptides and other biomolecules
  • Nonlinear emission by metallic nanoparticles/holes/antennas as potential nanophotonic devises
  • Stem cell differentiation and metabolic expression in a 3D tissue model for drug screening
  • Physico-chemical processes in fuel cell membranes

Link to official website http://www.finon-itn.eu/
​​
Work Package 1: Development of nonlinear and vibrational nanoscopy

To establish nonlinear and vibrational microscopy at the nano-level in order to study metallic nanostructures and macromolecular suprastructures without perturbing labelling molecules or sample preparation procedures.

 

Work Package Leader: Paola Borri
Work Package Students: Juris Kiskis, Michael Struhrenberg, Attilio Zilli, Naya Giannakopoulou, Marie Didier, Siyuan Wang, Alberto Lombardini, Carolina Rendon Barraza, Amala Elizabeth, Xiao Ling

Project Progress:
  • FWM epi-detected microscope with heterodyne interferometry implemented 
  
 
  • Dual-polarization phase-sensitive FWM detection implemented
  • Interface-sensitive wide-field SHG microscope implemented
  • Polarization resolved microscopy set-up for structural analysis of supramolecular assemblies and metallic nanoparticles, incorporating SHG, FWM, and Coherent Antistokes Raman Scattering (CARS), implemented
 
 
 Publications:

 



 




​Work Package 2: Fundamental studies of molecular suprastructures

To establish and compare the chemical sensitivity and spatial resolution of all nano-scale imaging approaches for the visualization of molecular suprastructures, primarily different kinds of amyloid peptides, oligomers and fibrils will be investigated.

Work Package Leader: Annika Enejder
Work Package Students: Juris Kiskis, Marie Didier, Siyuan Wang, Amala Elizabeth

Project Progress:
  • Nonlinear and nearfield nanoscopy and spectroscopy of molecular suprastructures in air; composition, orientation and symmetry of sub-units
    • Insulin beta amyloids in air: CARS microscopy images of larger insulin beta amyloid fibers 

 

CARS insulin beta amyloid fibers.jpg 
 
 

 

    • Microtubules in axons: SHG microscopy images of cortical neurons
 SHG microtubules neurons.jpg
 
​Work Package 3: Fundamental studies of metallic nanostructures

To investigate the nonlinear generation of light emission from metallic nanostructures and explore their use for nano-photonic devices and as tracing/sensing agents in cells, tissues and polymeric materials a series of fundamental studies on different nonlinear excitation schemes in nanoparticles, nanoholes, and nano-antennas will be conducted with the nonlinear nano-scale visualization techniques developed in WP 1.

Work Package Leader: Sophie Brasselet
Work Package Students: Michael Struhrenberg, Diana Ribeiro, Attilio Zilli, Naya Giannakopoulou, Carolina Rendon Barraza
 
Project Progress:
    • Four-wave mixing images of nanoparticles in cells

FWM nanoparticles.jpg 

​Work Package 4: Applications in sciences

​(1) To develop a protocol for drug screening in a 3D tissue model using nonlinear microscopy.

(2) To track nanoparticle endocytosis and endosomal escape at the single particle level to reveal receptor-mediated transport pathways for therapeutic delivery 

(3) To investigate the water behaviour inside fuel cell membrane for sustainable energy conversion

Work Package Leader: Anna Forslow (4.1), Paola Borri (4.2), Katrin Domke (4.3)
Work Package Students: Alexandra Paul, Diana Ribeiro, Alberto Lombardini, Carolina Rendon Barraza, Xiao Ling
 
Project Progress:
    • Nonlinear microscopy images of cells grown on protein engineered scaffolds

MSC on scaffolds.jpg 

    • Resonant Four-wave mixing microscopy of gold nanoparticles in cells correlative with fluorescence microscopy

Chalmers University of Technology

           
Alexandra Paul          Michael Struhrenberg

AstraZeneca

         

 Anna Forslow        Diana Ribeiro 

Cardiff University

                           
Paola Borri               Attilio Zilli                   Naya Gianakopoulou

Ecole Polytechnique Federale De Lausanne

                   
  Sylvie Roke           Marie EP Didier    

Institut Fresnel

                              
                   
Sophie Brasselet
      Alberto Lombardini  Carolina Rendon Barraza

Max Planck Institute for Polymer Research 

                    
 Katrin Domke           Amala Elizabeth           Xiao Ling

 
 

 Associated Partners and Mentees

  1. Accelopment AG (CH) - Jeanette Mueller
  2. APE Gmbh (DE) - Edlef Beuttner
    • Attilio Zilli
    • Michael Struhrenberg
  3. Hertz Training for Scientists (NL) - Brigitte Hertz
  4. Institut de Ciències Fotòniques (ES) - Romain Quidant
    • Vitalijs Zubkovs
    • Amala Elizabeth
  5. Lund University (SE) - Sara Linse
    • Alexandra Paul
  6. Nanonics Imaging Ltd. (IL) - Aaron Lewis
    • Juris Kiskis
  7. Polish Academy of Sciences (PL) - Wojciech Dzwolak
    • Alberto Lombardini
    • Naya Giannakopoulou
  8. VENTEON Laser Technologies GmbH (DE) - Thomas Binhammer
    • Xiao Ling
    • Marie Didier
  9. Friedrich Schiller University (DE) - Rachel Grange
    • Diana Ribeiro
    • Carolina Rendon



 

Funded by

  • European Commission (FP7) (Public, Belgium)

Calendar of Events

Management board meeting - March 2015 via Skype
Focus on Microscopy - May 2015
FINON workshop and Midterm reporting to EU at MPIP in Mainz - July 9-10 2015
Nonlinear optics course at EPFL (Sylvie) - July 20-24 2015

Publications

Hybrid Elastin-like Polypeptide–Polyethylene Glycol (ELP-PEG) Hydrogels with Improved Transparency and Independent Control of Matrix Mechanics and Cell Ligand Density. Huiyuan Wang , Lei Cai , Alexandra Paul , Annika Enejder , Sarah C. Heilshorn. Biomacromolecules. Vol. 15/Issue 9. September 2014

Probing Rotational and Translational Diffusion of Nanodoublers in Living Cells on Microsecond Time Scales. Carlos Macias-Romero , Marie E. P. Didier , Vitalijs Zubkovs , Lucas Delannoy , Fabrizia Dutto , Aleksandra Radenovic , Sylvie Roke. Nano Letters.  Vol. 14/Issue 5. May 2014.


High throughput second harmonic imaging for label-free biological applications. Carlos Macias-Romero , Marie E. P. Didier , Pascal Jourdain , Pierre Marquet , Pierre Magistretti , Orly B. Tarun , Vitalijs Zubkovs , Aleksandra Radenovic , Sylvie Roke. Optics Express. Vol. 22/Issue 25. January 2014.

Increased Adipogenesis of Human Adipose-Derived Stem Cells on Polycaprolactone Fiber Matrices. Cecilia Brannmark , Alexandra Paul , Diana Ribeiro , Bjorn Magnusson , Gabriella Brolon , Annika Enejder , Anna Forslow. PLoS One. Vol. 9/Issue 11. November 2014.

​Polymer brushes functionalized SiO2 nanoparticles based Nafion nanocomposites: A novel avenue to low-humidity proton conducting membranes. A. Farrukh, F. Ashraf, A. Kaltbeitzel, X. Ling, M. Wagner, H. Duran, A. Ghaffar, H. ur Rehman, S.H. Parekh, K.F. Domke, B. Yameen. Polymer Chemistry, 2015.

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Published: Thu 19 Jul 2018.