Every year hundreds of thousands of children in developing countries suffer from winter vomiting disease or related viral infections. The disease also hits the western world's health care services hard, closing departments and delaying treatments.
All viral infections are caused by an individual virus binding to specific receptors on the surface of a host cell. The thousands of copies of the virus which the host cell produces, quickly attack new cells and illness becomes inevitable. Early identification and understanding of how a virus binds to the cell's surface is vital in overcoming the disease.
Researchers at Chalmers and at the University of Gothenburg's Sahlgrenska Academy have now taken an important step towards both making diagnosis more effective and improving options for developing virus-inhibiting drugs. The results, soon to be published in the prestigious journal Physical Review Letters, are based on a method developed at Chalmers.
“Briefly, the method makes it possible to identify and study individual viruses, 40 nanometres in size. No other method, based on similar simple analysis, provides the same level of sensitivity without the virus having been modified in some way before the analysis,” says Professor Fredrik Höök who led the study.
At the Sahlgrenska Academy
, Professor Göran Larson has succeeded in identifying a number of sugar molecules which bind strongly to the particular virus that causes winter vomiting disease. This knowledge has now been combined with the methodology developed at Chalmers and the result is an opportunity to study in detail the very first contact between a virus and the surface of the cell which contains a number of different sugar molecules.
The increased level of sensitivity offered by this method may make it central to the assessment of drug candidates developed with the aim of preventing the virus from binding to its host cell.
By looking at the weak bindings which are the precursor to the strong interaction which causes the virus to be taken up by the cell, the researchers will also be able to study how the virus mutates year on year. These mutations are one of the causes of increased intensity of outbreaks, making quick diagnosis of new viral strains of vital importance.
Furthermore, as the individual virus can be identified, the researchers hope that it will be possible to attack the very small quantities of virus responsible for spreading the disease, e.g. via drinking water, at an earlier stage than is possible today.
The research is supported by Vinnova
, the Swedish Foundation for Strategic Research
and Chalmers’ Area of Advance Nanoscience and Nanotechnology
.Caption:The picture shows the structure of a norovirus (40 nm in diameter), the
one causing winter vomiting disease, with 12 of the 180 glycan-binding
sites indicted with green circles.
Image: Waqas Nasir, Sahlgrenska Academy, Sweden
Professor Fredrik Höök, Department of Applied Physics, Chalmers, +46 31 772 61 30, +46 70 895 12 39, firstname.lastname@example.org
Professor Göran Larson, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, +46 31 342 13 30, +46 70 625 02 16, email@example.com www.sahlgrenska.gu.se