Quantitative cell studies

Quantitative microscopy has in the last couple of years aroused substantial interest for life sciences applications. In eukaryotic cells, quantitative measurements of protein expression, protein localization and protein-protein interactions are key components for a proper understanding of protein functionality. FCC collaborates with the Center for Biophysical Imaging at Chalmers, for developing algorithms and computer software for quantitative in vivo imaging. A software for automated recognition and tracking of yeast cells from transmission microscope images, combined with quantification and localization of GFP-tagged proteins using fluorescence microscopy, is currently under development. For the image analysis algorithms used, the emphasis lies on robust methodologies which enables long time-lapse studies of protein localization, migration, and inheritance over several cell cycles, as well as high through-put screening of protein functionality of a large number of gene-disrupted cells.

Figure: Bright-field image and the corresponding fluorescence image of a GFP-tagged fluorescent protein in a population of yeast cells.

Published: Tue 05 Feb 2013.