Principles in fluorescence spectroscopy

  • Course code: FKBT115
  • Course higher education credits: 7.5
  • Department: CHEMISTRY AND CHEMICAL ENGINEERING
  • Graduate school: Chemistry
  • Course is normally given: Every third year
  • Language: The course will be given in English

The course deals with a general description of fluorescence and applications to it in chemical, physical, biochemical and medicinal contexts. Areas covered: General introduction to ground and excited states of molecules, instrumentation, fluorophores, excited state lifetimes, solvent effects, quenching, fluorescence anisotropy, energy transfer (FRET), multiphoton excitation, microscopy, single molecule detection, fluorescence correlation spectroscopy etc.

Examination
1) Written exam
2) Actively taking part in two instrument demonstrations (Steady-state and time-resolved fluorescence instrumentation)

Application information
Application is made by sending an email to Marcus Wilhelmsson (marcus.wilhelmsson@chalmers.se)

Prerequisites
Biophysical Chemistry (KFK021, FKBT160) or Applied Optical Spectroscopy (KFK150, FKBT165) or similar course at other Universities. The student should also in their course application write a project description about how they could use fluorescence in their main research project.

Literature
Principles in Fluorescence Spectroscopy, Third Edition
Joseph R. Lakowicz; Springer, New York, 2006
+ some extra material

Lecturers
Professors Marcus Wilhelmsson, Joakim Andréasson, Bo Albinsson, Maria Abrahamsson, and Fredrik Westerlund.
More information
Professor Marcus Wilhelmsson, email: marcus.wilhelmsson@chalmers.se, phone: +46 (0)31 772 30 51 Department of Chemistry and Chemical Engineering, Chemistry and Biochemistry, Kemivägen 10

 

Published: Fri 14 Dec 2012. Modified: Thu 20 Feb 2014