Bio-based production of adipic acid

: Emma Karlsson, Lisbeth Olsson, Valeria Mapelli, Jae Ho Shin and

Veronica Saez Jimenez

In this project the aim is to generate a microbial strain for biobased production of adipic acid (an important building block in the nylon industry) starting from renewable raw materials, such as forest residues.

Adipic acid is considered to be one of the most important dicarboxylic acids annually produced in the world. Today the production relies on fossil based non-renewable raw materials and during the process the very potent green house gas N2O is released. The aim with this project is to create a more sustainable and environmentally friendly process of adipic acid starting from renewable raw materials such as forest residues. To do so we are using biotechnological techniques to generating strains endowed with enzymes for conversion into adipic acid. The strategy is to use an already known biotechnological process for production of lysine and then convert lysine into adipic acid through a novel metabolic pathway. The pathway consists of four enzymatic steps where each reaction is known but for the first three steps there are today no known enzymes performing these reactions. The main focus is now to identify enzymes that can perform these reactions. This will include characterization of several candidate enzymes and also protein engineering to alter the specificity of the enzymes.

This project is part of BioBUF and is financed by FORMAS and Västra Götalandsregionen.


Published: Wed 08 Jul 2015. Modified: Thu 08 Dec 2016