Engineering of yeast for increased acetic acid tolerance

: Lina Lindahl, Lisbeth Olsson, Maurizio Bettiga

The aim of this project is to improve acetic acid tolerance in the industrially relevant yeast Saccharomyces cerevisiae. The high concentration of acetic acid released during pretreatment of lignocellulose raw material is a major obstacle to the microbial conversion of biomass to products in a biorefinery concept, emphasizing the urge to develop acetic acid tolerant microorganisms. 

Through extensive characterization of the acetic acid tolerant yeast Zygosaccharomyces bailii, we have identified the cell membrane as a target for strain engineering with potential to increase acetic acid tolerance in S. cerevisiae. Ongoing work focuses on reducing acetic acid membrane permeability though engineering of the lipid metabolism.
This project is financially supported by Energimyndigheten, VR and the Chalmers Area of Advance Energy.



Published: Wed 08 Jul 2015. Modified: Tue 31 May 2016